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One-step, rapid fluorescence sensing of fungal viability based on a bioprobe with aggregation-induced emission characteristics

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Abstract

Fungi play key roles in various fields, including fermentation-based industries, medicine, and public health. The rapid and precise evaluation of fungal viability is critical for fungi-related fields. However, the methods currently applied have a series of limitations, such as time-consuming, laborious, and cumbersome. In this study, we designed and synthesized a water-dispersible fluorescent probe DPASI with typical aggregation-induced emission (AIE) characteristics for selectively lighting up dead Candida cells within 5 minutes. The rapid staining of the dead Candida cells could be ascribed to the efficient binding of DPASI with mitochondria, which was revealed by high-resolution fluorescence images taken by a structured illumination microscope (SIM) and colocalization images taken by a confocal laser scanning microscope (CLSM). Compared with traditional probes for the identification of dead Candida cells, the DPASI probe does not require tedious centrifugation and washing steps and has significant advantages in time-and-labor-saving. It thus provides a facile and promising tool for the rapid evaluation of antifungal susceptibility and screening of new antifungal drugs, which would greatly contribute to the fungal research.

Graphical abstract: One-step, rapid fluorescence sensing of fungal viability based on a bioprobe with aggregation-induced emission characteristics

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Article information


Submitted
03 Dec 2019
Accepted
17 Jan 2020
First published
20 Jan 2020

Mater. Chem. Front., 2020, Advance Article
Article type
Research Article

One-step, rapid fluorescence sensing of fungal viability based on a bioprobe with aggregation-induced emission characteristics

X. Ge, M. Gao, B. Situ, W. Feng, B. He, X. He, S. Li, Z. Ou, Y. Zhong, Y. Lin, X. Ye, X. Hu, B. Z. Tang and L. Zheng, Mater. Chem. Front., 2020, Advance Article , DOI: 10.1039/C9QM00732F

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