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Issue 18, 2020
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Anticancer potency of novel organometallic Ir(iii) complexes with phosphine derivatives of fluoroquinolones encapsulated in polymeric micelles

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Abstract

Novel half-sandwich iridium(III) complexes with aminomethyl(diphenyl)phosphine derived from fluoroloquinolones (IrPCp, IrPSf, IrPLm, IrPNr) were studied as possible anticancer chemotherapeutics and showed higher potency than other well-known metal-based agents i.e., Pt(II) drugs. All compounds were characterized by elemental analysis, selected spectroscopic methods (i.e., absorption and fluorescence spectroscopy, NMR), ESI-MS spectrometry, X-ray diffraction, and electrochemical techniques. The studied complexes exhibited promising cytotoxicity in vitro with IC50 values significantly lower than that of the reference drug – cisplatin. The insight into the mode of action revealed the uniform distribution of the Ir(III) complexes in both the nucleus and cytoplasm (Pearson's co-localization coefficient of 0.63). Precise cytometric analysis provided clear evidence for the predominance of apoptosis in the induced cell death. The activation of caspase-3/7 along with the decrease of mitochondrial membrane potential also confirmed the apoptotic cell death. The investigated Ir(III) complexes may induce changes in the cell cycle leading to G2/M phase arrest. ROS generation as a plausible pathway responsible for the cytotoxicity was confirmed by determination of redox potentials enabling efficient ROS production. Furthermore, Pluronic P-123 micelles loaded with selected Ir(III) complexes were proposed to overcome low solubility and to minimize serious systemic side effects by administering the complex in a controlled manner. The resulting nanoformulations (IrPCp_M, IrPNr_M) facilitated efficient drug accumulation for human lung adenocarcinoma and human prostate carcinoma (A549 and DU-145 cell lines), as demonstrated by confocal microscopy and ICP-MS analysis. In vitro cytotoxicity assays were also carried out within multicellular tumor spheroids and efficient anticancer action on these 3D assemblies was demonstrated.

Graphical abstract: Anticancer potency of novel organometallic Ir(iii) complexes with phosphine derivatives of fluoroquinolones encapsulated in polymeric micelles

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Supplementary files

Article information


Submitted
07 May 2020
Accepted
20 Jul 2020
First published
23 Jul 2020

Inorg. Chem. Front., 2020,7, 3386-3401
Article type
Research Article

Anticancer potency of novel organometallic Ir(III) complexes with phosphine derivatives of fluoroquinolones encapsulated in polymeric micelles

S. Kozieł, U. K. Komarnicka, A. Ziółkowska, A. Skórska-Stania, B. Pucelik, M. Płotek, V. Sebastian, A. Bieńko, G. Stochel and A. Kyzioł, Inorg. Chem. Front., 2020, 7, 3386
DOI: 10.1039/D0QI00538J

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