Issue 9, 2020

A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells

Abstract

The two-photon absorption properties of a pyrene-pyridinium dye (1) were studied for potential application in two-photon spectroscopy. When probe 1 was used in cellular two-photon fluorescence microscopy imaging, it allowed the visualization of nuclei in live cells with a relatively low probe concentration (such as 1 μM). Spectroscopic evidence further revealed that probe 1 interacted with DNA as an intercalator. The proposed DNA intercalation properties of probe 1 were consistent with the experimental findings that suggested that the observed nucleus staining ability is dependent on the substituents on the pyridinium fragment of the probe.

Graphical abstract: A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells

Supplementary files

Article information

Article type
Paper
Submitted
22 Mar 2020
Accepted
09 Jun 2020
First published
19 Jun 2020

Photochem. Photobiol. Sci., 2020,19, 1152-1159

A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells

C. S. Abeywickrama, K. J. Wijesinghe, C. B. Plescia, L. S. Fisher, T. Goodson III, R. V. Stahelin and Y. Pang, Photochem. Photobiol. Sci., 2020, 19, 1152 DOI: 10.1039/D0PP00107D

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