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Issue 2, 2021
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Mechanistic divergence between (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol synthases from Dictyostelium purpureum and Streptomyces coelicolor

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Abstract

The main product of DpTPS9 from the social amoeba Dictyostelium purpureum was identified as (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol that is also known as an intermediate of bacterial geosmin synthase, but the experimentally verified cyclisation mechanisms differ. Together with the low sequence identity this points to convergent evolution. The functionality of selected residues in DpTPS9 was investigated via site-directed mutagenesis experiments.

Graphical abstract: Mechanistic divergence between (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol synthases from Dictyostelium purpureum and Streptomyces coelicolor

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Article information


Submitted
26 Nov 2020
Accepted
15 Dec 2020
First published
15 Dec 2020

Org. Biomol. Chem., 2021,19, 370-374
Article type
Communication

Mechanistic divergence between (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol synthases from Dictyostelium purpureum and Streptomyces coelicolor

H. Xu, J. Rinkel, X. Chen, T. G. Köllner, F. Chen and J. S. Dickschat, Org. Biomol. Chem., 2021, 19, 370
DOI: 10.1039/D0OB02361B

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