Live-cell Imaging of Lipid Droplets by Solvatochromic Styrycoumarin Derivatives
Lipid droplets (LDs), the lipid-rich intracellular organelles, serve to regulate many physiological processes and therefore attracted attention towards selective detection of lipid droplets. We report positively solvatochromic lipophilic dyes, based on the push-pull framework containing coumarin-pyridine heterocycles for selective live-cell imaging of lipid droplets (LDs) in Cos-7 and McA-RH7777 cells at ultralow concentrations (200 nM). The fluorescence probes show a remarkable increase in fluorescence intensity with time with the hydrophobic core of the lipid droplets contributing to the observed intensity enhancement. The simple structural framework, red-emission, strong Stokes shift (>80 nm), excellent biocompatibility highlights their significance as a versatile imaging tool for studying of Lipid droplets (LDs).
- This article is part of the themed collection: Chemical biology in OBC