Jump to main content
Jump to site search


An enzyme-triggered turn-on fluorescent probe based on carboxylate-induced detachment of a fluorescence quencher

Author affiliations

Abstract

We developed a new class of turn-on fluorescent probes for an esterase. After the esterase-mediated hydrolysis produced carboxylate (as a fluorescence activator), the fluorescence intensity was markedly increased through the detachment of a quencher moiety from the quenched Cy5 fluorophore. Because the probes based on this new activator-induced quencher-detachment (AiQd) adopt a non-immolative linker between the cleavable site and the fluorophore, the rate of the enzymatic reaction is greatly improved, without the generation of any by-products.

Graphical abstract: An enzyme-triggered turn-on fluorescent probe based on carboxylate-induced detachment of a fluorescence quencher

Back to tab navigation

Supplementary files

Article information


Submitted
30 Apr 2020
Accepted
17 Aug 2020
First published
18 Aug 2020

Org. Biomol. Chem., 2020, Advance Article
Article type
Communication

An enzyme-triggered turn-on fluorescent probe based on carboxylate-induced detachment of a fluorescence quencher

M. Oe, K. Miki and K. Ohe, Org. Biomol. Chem., 2020, Advance Article , DOI: 10.1039/D0OB00899K

Social activity

Search articles by author

Spotlight

Advertisements