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Issue 15, 2020
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Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina

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Abstract

A synthetic biology approach based on genome mining and heterologous biosynthesis is a powerful tool for discovering novel natural products from a tremendous gene resource. We carried out fungal genome mining guided by a polyketide synthase gene using a public database and found a putative macrolide biosynthetic gene cluster with a highly reducing polyketide synthase gene and a thioesterase gene in Macrophomina phaseolina. Reconstitution of the cluster in Aspergillus oryzae, a model heterologous host for fungal natural product biosynthesis, produced a new 12-membered macrolide, phaseolide A. The absolute stereochemistry was elucidated by vibrational circular dichroism spectroscopy and the crystalline sponge method.

Graphical abstract: Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina

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Submitted
11 Mar 2020
Accepted
19 Mar 2020
First published
19 Mar 2020

Org. Biomol. Chem., 2020,18, 2813-2816
Article type
Communication

Synthetic-biology-based discovery of a fungal macrolide from Macrophomina phaseolina

Y. Morishita, T. Sonohara, T. Taniguchi, K. Adachi, M. Fujita and T. Asai, Org. Biomol. Chem., 2020, 18, 2813
DOI: 10.1039/D0OB00519C

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