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Issue 16, 2020
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Biochemical characterisation of an α1,4 galactosyltransferase from Neisseria weaveri for the synthesis of α1,4-linked galactosides

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Abstract

The human cell surface trisaccharide motifs globotriose and P1 antigen play key roles in infections by pathogenic bacteria, which makes them important synthetic targets as antibacterial agents. Enzymatic strategies to install the terminal α1,4-galactosidic linkage are very attractive but have only been demonstrated for a limited set of analogues. Herein, a new bacterial α1,4 galactosyltransferase from N. weaveri was cloned and produced recombinantly in E. coli BL21 (DE3) cells, followed by investigation of its substrate specificity. We demonstrate that the enzyme can tolerate galactosamine (GalN) and also 6-deoxygalactose and 6-deoxy-6-fluorogalactose as donors, and lactose and N-acetyllactosamine as acceptors, leading directly to analogues of Gb3 and P1 that are valuable chemical probes and showcase how biocatalysis can provide fast access to a number of unnatural carbohydrate analogues.

Graphical abstract: Biochemical characterisation of an α1,4 galactosyltransferase from Neisseria weaveri for the synthesis of α1,4-linked galactosides

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Supplementary files

Article information


Submitted
24 Feb 2020
Accepted
30 Mar 2020
First published
30 Mar 2020

Org. Biomol. Chem., 2020,18, 3142-3148
Article type
Paper

Biochemical characterisation of an α1,4 galactosyltransferase from Neisseria weaveri for the synthesis of α1,4-linked galactosides

K. Huang, A. Marchesi, K. Hollingsworth, P. Both, A. P. Mattey, E. Pallister, H. Ledru, S. J. Charnock, M. C. Galan, W. B. Turnbull, F. Parmeggiani and S. L. Flitsch, Org. Biomol. Chem., 2020, 18, 3142
DOI: 10.1039/D0OB00407C

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