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Lysosome-Targeted Near-infrared Fluorescent Probe for Imaging of Acid Phosphatase in Living Cells

Abstract

Fluorescence probe for the detection of acid phosphatases (ACP) is important for the investigation of pathology and diagnosis of diseases. We reported a lysosome-targeted near-infrared (NIR) fluorescent probe SHCy-P based on a novel NIR-emitting thioxanthene−indolium dye for ACP. The probe showed a long wavelength fluorescence emission at λem = 765 nm. Due to the ACP-catalyzed cleavage of the phosphate group in SHCy-P, the probe exhibited high selectivity and sensitivity for the 'turn-on' detection of ACP with a limit of detection as low as 0.48 U/L. The probe SHCy-P could also be used to detect and image endogenous ACP in lysosomes. In light of these prominent properties, we envision that SHCy-P will be an efficient optical imaging approach for investigating ACP activity in disease diagnosis.

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Supplementary files

Article information


Submitted
09 Oct 2019
Accepted
14 Jan 2020
First published
15 Jan 2020

Org. Biomol. Chem., 2020, Accepted Manuscript
Article type
Paper

Lysosome-Targeted Near-infrared Fluorescent Probe for Imaging of Acid Phosphatase in Living Cells

X. Zeng, S. Cai, C. Liu, X. Jiao, S. He and L. Zhao, Org. Biomol. Chem., 2020, Accepted Manuscript , DOI: 10.1039/C9OB02188D

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