Jump to main content
Jump to site search

Issue 42, 2020
Previous Article Next Article

High-throughput nitrogen-vacancy center imaging for nanodiamond photophysical characterization and pH nanosensing

Author affiliations

Abstract

The fluorescent nitrogen-vacancy (NV) defect in diamond has remarkable photophysical properties, including high photostability which allows stable fluorescence emission for hours; as a result, there has been much interest in using nanodiamonds (NDs) for applications in quantum optics and biological imaging. Such applications have been limited by the heterogeneity of NDs and our limited understanding of NV photophysics in NDs, which is partially due to the lack of sensitive and high-throughput methods for photophysical analysis of NDs. Here, we report a systematic analysis of NDs using two-color wide-field epifluorescence imaging coupled to high-throughput single-particle detection of single NVs in NDs with sizes down to 5–10 nm. By using fluorescence intensity ratios, we observe directly the charge conversion of single NV center (NV or NV0) and measure the lifetimes of different NV charge states in NDs. We also show that we can use changes in pH to control the main NV charge states in a direct and reversible fashion, a discovery that paves the way for performing pH nanosensing with a non-photobleachable probe.

Graphical abstract: High-throughput nitrogen-vacancy center imaging for nanodiamond photophysical characterization and pH nanosensing

Back to tab navigation

Supplementary files

Article information


Submitted
12 Aug 2020
Accepted
14 Sep 2020
First published
26 Oct 2020

This article is Open Access

Nanoscale, 2020,12, 21821-21831
Article type
Paper

High-throughput nitrogen-vacancy center imaging for nanodiamond photophysical characterization and pH nanosensing

M. Sow, H. Steuer, S. Adekanye, L. Ginés, S. Mandal, B. Gilboa, O. A. Williams, J. M. Smith and A. N. Kapanidis, Nanoscale, 2020, 12, 21821
DOI: 10.1039/D0NR05931E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements