Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 28, 2020
Previous Article Next Article

Dissecting the intracellular signalling and fate of a DNA nanosensor by super-resolution and quantitative microscopy

Author affiliations

Abstract

DNA nanodevices have been developed as platforms for the manipulation of gene expression, delivery of molecular payloads, and detection of various molecular targets within cells and in other complex biological settings. Despite efforts to translate DNA nanodevices from the test tube (in vitro) to living cells, their intracellular trafficking and functionality remain poorly understood. Herein, quantitative and super-resolution microscopy approaches were employed to track and visualise, with nanometric resolution, the molecular interactions between a synthetic DNA nanosensor and transcription factors in intracellular compartments. Specifically, fluorescence resonance energy transfer microscopy, fluorescence correlation spectroscopy, fluorescence lifetime imaging microscopy and multicolour single-molecule localisation microscopy were employed to probe the specific binding of the DNA nanosensor to the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). We monitored the mobility, subcellular localisation and degradation of the DNA nanosensor inside living prostate cancer PC3 cells. Super-resolution imaging enabled the direct visualisation of the molecular interactions between the synthetic DNA nanosensors and the NF-κB molecules in cells. This study represents a significant advance in the effective detection as well as understanding of the intracellular dynamics of DNA nanosensors in a complex biological milieu.

Graphical abstract: Dissecting the intracellular signalling and fate of a DNA nanosensor by super-resolution and quantitative microscopy

Back to tab navigation

Supplementary files

Article information


Submitted
20 Apr 2020
Accepted
01 Jul 2020
First published
01 Jul 2020

Nanoscale, 2020,12, 15402-15413
Article type
Paper

Dissecting the intracellular signalling and fate of a DNA nanosensor by super-resolution and quantitative microscopy

A. Glab, A. Bertucci, F. Martino, M. Wojnilowicz, A. Amodio, M. Venanzi, F. Ricci, G. Forte, F. Caruso and F. Cavalieri, Nanoscale, 2020, 12, 15402
DOI: 10.1039/D0NR03087B

Social activity

Search articles by author

Spotlight

Advertisements