Nanophotonics based label free detection mechanism for real-time monitoring of interleukin-6
Magneto-photonic crystals/MPCs are promising candidates for devising high-fidelity embedded biosensor system which offers facile & real time detection of diagnostic proteins. Despite of extensive use of magnetic nanomaterials for theranostic applications, the idea of exploiting its photonic response when assembled as colloid inside a matrix remained unexplored. Herein, we report a novel label free method for quantitative detection of Interleukin 6 which is a widely used prognostic marker for multiple pathological conditions. Cobalt Ferrite/CoF and magnetite nanoparticles with Ms of 74.8 and 77 emu/g were assembled inside a hydrogel matrix with the application of an external magnetic field. Through the use of click chemistry, detecting antibodies were immobilized on their surface. The interaction of interleukin 6 with antibody, produces a blue-shift in resonant wavelength and the reflectance intensity increases up to 50 % and 44 % when tested with CoF & Magnetite based MPC respectively at a concentration of 50 pg/ml. The dynamic range of sensor lies within the prognostic values of IL-6 and the integrated sensing mechanism proposed in this study, provides an ideal platform for real-time management of sepsis in patients with higher degree burns.