Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 26, 2020
Previous Article Next Article

An electrochemical biosensor exploiting binding-induced changes in electron transfer of electrode-attached DNA origami to detect hundred nanometer-scale targets

Author affiliations

Abstract

The specific detection in clinical samples of analytes with dimensions in the tens to hundreds of nanometers, such as viruses and large proteins, would improve disease diagnosis. Detection of these “mesoscale” analytes (as opposed to their nanoscale components), however, is challenging as it requires the simultaneous binding of multiple recognition sites often spaced over tens of nanometers. In response, we have adapted DNA origami, with its unparalleled customizability to precisely display multiple target-binding sites over the relevant length scale, to an electrochemical biosensor platform. Our proof-of-concept employs triangular origami covalently attached to a gold electrode and functionalized with redox reporters. Electrochemical interrogation of this platform successfully monitors mesoscale, target-binding-induced changes in electron transfer in a manner consistent with coarse-grained molecular dynamics simulations. Our approach enables the specific detection of analytes displaying recognition sites that are separated by ∼40 nm, a spacing significantly greater than that achieved in similar sensor architectures employing either antibodies or aptamers.

Graphical abstract: An electrochemical biosensor exploiting binding-induced changes in electron transfer of electrode-attached DNA origami to detect hundred nanometer-scale targets

Back to tab navigation

Supplementary files

Article information


Submitted
04 Feb 2020
Accepted
17 Jun 2020
First published
18 Jun 2020

Nanoscale, 2020,12, 13907-13911
Article type
Communication

An electrochemical biosensor exploiting binding-induced changes in electron transfer of electrode-attached DNA origami to detect hundred nanometer-scale targets

N. Arroyo-Currás, M. Sadeia, A. K. Ng, Y. Fyodorova, N. Williams, T. Afif, C. Huang, N. Ogden, R. C. Andresen Eguiluz, H. Su, C. E. Castro, K. W. Plaxco and P. S. Lukeman, Nanoscale, 2020, 12, 13907
DOI: 10.1039/D0NR00952K

Social activity

Search articles by author

Spotlight

Advertisements