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Ca2+ participating self-assembly of an apoferritin nanostructure for nucleic acid drug delivery

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Abstract

One of the most encountered obstacles for utilizing nano-sized vehicles to implement the in vivo delivery of nucleic acid drugs (NADs) is the possible steric hindrance caused by their intrinsic size and charge. In this work, we added Ca2+ for the pH triggered self-assembly process of H-apoferritin (HFn), to neutralize negative charges and help siRNA condense during complexation and particle formation. As expected, the internalization efficiency of siRNA in HFn particle formation could be enhanced 1.65-fold, compared with that without incorporated Ca2+. Furthermore, the calcification that occurred within the cavity of HFn particles endows them with endosomal escape capability, which could explain their contribution to the demonstrated in vitro and in vivo gene silencing effect achieved by the internalized siRNA. Thus, this Ca2+ participating self-assembly process of a protein nanostructure would lead to advanced internalization efficiency for NAD therapy.

Graphical abstract: Ca2+ participating self-assembly of an apoferritin nanostructure for nucleic acid drug delivery

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Article information


Submitted
20 Jan 2020
Accepted
23 Feb 2020
First published
24 Mar 2020

Nanoscale, 2020, Advance Article
Article type
Paper

Ca2+ participating self-assembly of an apoferritin nanostructure for nucleic acid drug delivery

H. Huang, K. Sha, H. Veroniaina, Z. Wu, Z. Wu and X. Qi, Nanoscale, 2020, Advance Article , DOI: 10.1039/D0NR00547A

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