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Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

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Abstract

Super-resolution optical fluctuation imaging (SOFI) produces fast, background-free, super-resolved images by analyzing the temporal fluorescence fluctuations of independent emitters. With sufficient brightness and fluctuations, a higher order of image processing affords a higher resolution and in principle the resolution enhancement is unbounded. However, it is practically challenging to find suitable probes for high-order SOFI. Herein, we report two types of BODIPY-based polymer dots (Pdots) with narrow-band emissions, pronounced fluctuations, and prominent photostability, thus enabling high-order, dual-color SOFI nanoscopy. Single-particle and subcellular SOFI analysis reveals the superior performance of the BODIPY Pdots as compared to conventional streptavidin-conjugated Alexa dyes. In contrast with wide-field images, the spatial resolution (∼57 nm) was enhanced by ∼6.0-fold in 8th-order single-particle SOFI nanoscopy. A spatial resolution (61 nm) was obtained for single microtubules labeled by the BODIPY Pdots, while the majority of the subcellular structures were lost for those labeled by streptavidin-Alexa dyes in 8th-order SOFI. This work indicates the unprecedented performance of Pdot probes for multi-color subcellular SOFI applications.

Graphical abstract: Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

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Supplementary files

Article information


Submitted
13 Jan 2020
Accepted
16 Mar 2020
First published
18 Mar 2020

Nanoscale, 2020, Advance Article
Article type
Communication

Narrow-band polymer dots with pronounced fluorescence fluctuations for dual-color super-resolution imaging

Z. Liu, J. Liu, Z. Zhang, Z. Sun, X. Shao, J. Guo, L. Xi, Z. Yuan, X. Zhang, D. T. Chiu and C. Wu, Nanoscale, 2020, Advance Article , DOI: 10.1039/D0NR00347F

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