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Issue 12, 2020
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Accurate detection of β-hCG in women's serum and cervical secretions for predicting early pregnancy viability based on time-resolved luminescent lanthanide nanoprobes

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Abstract

Sensitive and specific detection of β-hCG in women's serum and cervical secretions is of great significance for early pregnancy evaluation. However, the accurate detection of trace amounts of β-hCG in cervical secretions remains challenging because of its low level. Herein, we report a unique strategy for β-hCG detection in a heterogeneous sandwich-type bioassay by using LiLuF4:Ce,Tb nanoparticles as time-resolved photoluminescence (PL) nanoprobes. By taking advantage of the intense and long-lived PL of the nanoprobes, the short-lived background autofluorescence can be completely eliminated, which enables the sensitive detection of β-hCG with a linear range of 0–10 ng mL−1 and a detection limit down to 6.1 pg mL−1, approximately two orders of magnitude improvement relative to that of a commercial β-hCG assay kit. Furthermore, we demonstrate the application of the nanoprobes for accurate detection of β-hCG in clinical serum and cervical secretion samples and unveil that the ratio of β-hCG levels in cervical secretions and serum can be a good indicator of early pregnancy viability in unknown locations. These findings bring new opportunities in perinatal medicine by employing luminescent lanthanide nanoprobes, thus laying a foundation for future development of luminescent nanoprobes for versatile biomedical applications.

Graphical abstract: Accurate detection of β-hCG in women's serum and cervical secretions for predicting early pregnancy viability based on time-resolved luminescent lanthanide nanoprobes

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Supplementary files

Article information


Submitted
31 Dec 2019
Accepted
16 Feb 2020
First published
18 Feb 2020

Nanoscale, 2020,12, 6729-6735
Article type
Paper

Accurate detection of β-hCG in women's serum and cervical secretions for predicting early pregnancy viability based on time-resolved luminescent lanthanide nanoprobes

L. Cai, Y. Huang, P. Sun, W. Zheng, S. Zhou, P. Huang, J. Wei, D. Tu, X. Chen and Z. Liang, Nanoscale, 2020, 12, 6729
DOI: 10.1039/C9NR10973K

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