Jump to main content
Jump to site search


An ovarian spheroid based tumor model that represents vascularized tumors and enables the investigation of nanomedicine therapeutics

Author affiliations

Abstract

The failure of cancer therapies in clinical settings is often attributed to the lack of a relevant tumor model and pathological heterogeneity across tumor types in the clinic. The objective of this study was to develop a robust in vivo tumor model that better represents clinical tumors for the evaluation of anti-cancer therapies. We successfully developed a simple mouse tumor model based on 3D cell culture by injecting a single spheroid and compared it to a tumor model routinely used by injecting cell suspension from 2D monolayer cell culture. We further characterized both tumors with cellular markers for the presence of myofibroblasts, pericytes, endothelial cells and extracellular matrix to understand the role of the tumor microenvironment. We further investigated the effect of chemotherapy (doxorubicin), nanomedicine (Doxil®), biological therapy (Avastin®) and their combination. Our results showed that the substantial blood vasculature in the 3D spheroid model enhances the delivery of Doxil® by 2.5-fold as compared to the 2D model. Taken together, our data suggest that the 3D tumors created by simple subcutaneous spheroid injection represents a robust and more vascular murine tumor model which is a clinically relevant platform to test anti-cancer therapy in solid tumors.

Graphical abstract: An ovarian spheroid based tumor model that represents vascularized tumors and enables the investigation of nanomedicine therapeutics

Back to tab navigation

Supplementary files

Article information


Submitted
09 Nov 2019
Accepted
18 Dec 2019
First published
19 Dec 2019

Nanoscale, 2020, Advance Article
Article type
Paper

An ovarian spheroid based tumor model that represents vascularized tumors and enables the investigation of nanomedicine therapeutics

M. S. Singh, M. Goldsmith, K. Thakur, S. Chatterjee, D. Landesman-Milo, T. Levy, L. A. Kunz-Schughart, Y. Barenholz and D. Peer, Nanoscale, 2020, Advance Article , DOI: 10.1039/C9NR09572A

Social activity

Search articles by author

Spotlight

Advertisements