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A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification

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Abstract

Exosomes are membrane nanovesicles carrying molecular information that may reflect the biological and genetic characteristics of their parent cells. Numerous studies have demonstrated the potential of exosomes as noninvasive cancer biomarkers. Hence, specific detection of cancer cell-derived exosomes is of significant importance. Here, we developed a fluorescence assay for the determination of gastric cancer exosomes based on branched rolling circle amplification (BRCA) and an aptamer to target specific exosomes. The designed padlock probe was cyclized after incubation with an aptamer binding with the target exosome. BRCA was triggered by adding a second primer and the resulting long tandem double-stranded DNA product was detected using SYBR Green I as the fluorescent dye. This method demonstrated a high specificity for target exosomes with a detection limit of 4.27 × 104 exosomes per mL. Moreover, plasma from gastric cancer patients was tested to verify the clinical applicability of this assay. Our results demonstrated that this aptamer-based biosensor may show potential for the early diagnosis of gastric cancer.

Graphical abstract: A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification

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Article information


Submitted
11 Oct 2019
Accepted
02 Dec 2019
First published
03 Dec 2019

Nanoscale, 2020, Advance Article
Article type
Paper

A simple fluorescence aptasensor for gastric cancer exosome detection based on branched rolling circle amplification

R. Huang, L. He, S. Li, H. Liu, L. Jin, Z. Chen, Y. Zhao, Z. Li, Y. Deng and N. He, Nanoscale, 2020, Advance Article , DOI: 10.1039/C9NR08747H

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