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Issue 3, 2020
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An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

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Abstract

Ribonuclease H (RNase H), an intracellular ribonuclease, plays a crucial role in cellular processes and especially relates to many disease processes. Here, we report a novel signal amplification strategy based on an RNase H-powered DNA walking machine for specific and sensitive RNase H activity detection. The DNA walking machine is composed of a small quantity of DNA walker strands and abundant FAM-labeled DNA–RNA chimeric strands on a single gold nanoparticle (AuNP). RNase H can specifically degrade the RNA fragment in a DNA–RNA hybrid duplex and trigger the autonomous movement of a DNA walker strand on the AuNP surface. During this process, each step of the walking can release the FAM-labeled RNA from the surface of the AuNP, realizing the signal amplification for RNase H sensing. This method has been successfully utilized for RNase H activity detection in a complex system and applied for screening of related inhibitors. Therefore, our RNase H-powered DNA walking machine gives a novel platform for RNase H activity detection and RNase H-associated drug discovery.

Graphical abstract: An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

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Article information


Submitted
01 Sep 2019
Accepted
03 Dec 2019
First published
04 Dec 2019

Nanoscale, 2020,12, 1673-1679
Article type
Paper

An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

Y. Wang, N. Hu, C. Liu, C. Nie, M. He, J. Zhang, Q. Yu, C. Zhao, T. Chen and X. Chu, Nanoscale, 2020, 12, 1673
DOI: 10.1039/C9NR07550J

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