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Simple and Fast Protocol for Synthesis of 2-amino-4-(4-formylphenyl)-4H-chromene-3-carbonitrile (AFC) to Developed an Optical Immunoassay for Quantification of Botulinum neurotoxin type F (BoNT/F)

Abstract

In this work, a novel optical immunoassay platform using(S)-2-amino-4-(4-formylphenyl)-4H-chromene-3-carbonitrile as an optical probe which was synthesized by an ultra-sonication method. The structure of the probe was elucidated by Fourier-transform infrared spectroscopy and Nuclear magnetic resonance spectroscopy spectral techniques. The physicochemical properties of the probe were analyzed by Ultraviolet-Visible and Fluorescent spectroscopy. This probe was attached with antibody through the 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-Hydroxysuccinimide coupling using new amide linkage forming and successfully applied for the detection of Botulinum neurotoxin type F. Under the optimized condition, the immunoassay shows a linear range for Botulinum neurotoxin type Ffrom 50 to 500 fg/mL and Limit of Detection 34.4 fg/mL. The simple fluorescent sensor detected Botulinum neurotoxin type F through the Twisted Intramolecular Charge Transfer and Static quenching mechanisms. This is the first optical immunoassay for the detection of Botulinum neurotoxin type F and it has a wide range of sensitivity, good selectivity, less time consuming and low cost. Finally, the immunoassay was applied for different food samples. Keywords: Botulinum-F, (S)-2-amino-4-(4-formylphenyl)-4H-chromene-3- carbonitrile (AFC), Biosensor, Limit of Detection (LOD), selectivity studies, food samples, Immunoassay

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Supplementary files

Article information


Submitted
14 Aug 2020
Accepted
10 Oct 2020
First published
13 Oct 2020

New J. Chem., 2020, Accepted Manuscript
Article type
Paper

Simple and Fast Protocol for Synthesis of 2-amino-4-(4-formylphenyl)-4H-chromene-3-carbonitrile (AFC) to Developed an Optical Immunoassay for Quantification of Botulinum neurotoxin type F (BoNT/F)

K. Shenbagavalli, S. K. Yadav, P. Ananthappan, S. Ellairaja, P. Sarkaraisamy and V. S. pandian, New J. Chem., 2020, Accepted Manuscript , DOI: 10.1039/D0NJ04103C

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