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Comparison of molecularly imprinted plasmonic nanosensor performances for bacteriophage detection

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Abstract

Contamination of drinking and surface waters with microorganisms is a problem that attracts attention because it threatens human health. One of the microorganisms that determine fecal contamination in water is a bacteriophage that infects coliform bacteria. A fast, real-time, sensitive, and low-cost alternative method is the nanosensor system for microorganism detection. This study aimed to develop a surface plasmon resonance nanosensor for the detection of T4 bacteriophages using nanoparticle- and nanofilm-based polymers. In this context, nanoparticle and nanofilm-based imprinted polymers were synthesized to develop nanosensors, and their efficacy was compared in fecal pollution determination in water. Following the characterization studies, nanoparticle and nanofilm-based plasmonic nanosensors were used to carry out kinetic studies in the 1 × 104–4 × 106 pfu mL−1 concentration range in buffer, sea, and tap water sample solutions. Reusability and selectivity studies were also performed. According to the results, the nanoparticle and nanofilm-based plasmonic nanosensors could measure with 98% and 81% precision, respectively. The limit of detection values was calculated as 6 × 103 pfu mL−1 and 8 × 103 pfu mL−1 for the nanoparticle and the nanofilm-based plasmonic nanosensors, respectively. In addition, the recovery value of the nanoparticle-based plasmonic nanosensor was calculated as 91–96%, while the nanofilm-based plasmonic nanosensor was calculated as 85–90% in tap and seawater samples. All results showed that both plasmonic nanosensors could detect with high selectivity, but the nanoparticle-based plasmonic nanosensor has higher sensitivity than the nanofilm-based plasmonic nanosensor.

Graphical abstract: Comparison of molecularly imprinted plasmonic nanosensor performances for bacteriophage detection

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Supplementary files

Article information


Submitted
11 Aug 2020
Accepted
21 Sep 2020
First published
21 Sep 2020

New J. Chem., 2020, Advance Article
Article type
Paper

Comparison of molecularly imprinted plasmonic nanosensor performances for bacteriophage detection

Ö. Erdem, N. Cihangir, Y. Saylan and A. Denizli, New J. Chem., 2020, Advance Article , DOI: 10.1039/D0NJ04053C

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