Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 26, 2020
Previous Article Next Article

A molecular rotor sensor for detecting mitochondrial viscosity in apoptotic cells by two-photon fluorescence lifetime imaging

Author affiliations

Abstract

Impairing mitochondrial function may change its viscosity. The detection of changes in the mitochondrial viscosity is of great significance for biomedical research. Herein, we have designed a D–π–A type two-photon fluorescent probe ((4-(bis(4-(pyridin-4-yl)phenyl)amino)styryl)-1-methylpyridin-1-ium iodide) (DPTPA-Py) with a triphenylamine derivative as the electron donor and pyridinium as the electron acceptor for detecting mitochondrial viscosity in living cells. It is found that both fluorescence intensity and fluorescence lifetime of DPTPA-Py are viscosity-dependent, and the probe exhibits a strong anti-interference ability in the detection of viscosity. The colocalization experiments reveal that DPTPA-Py is located in mitochondria only and is highly biocompatible. The probe shows no significant effects on the mitochondrial membrane potential. In addition, the probe is successfully used to monitor mitochondrial viscosity changes during apoptosis of living cells by two-photon microscopy (TPM) and fluorescence lifetime imaging microscopy (FLIM).

Graphical abstract: A molecular rotor sensor for detecting mitochondrial viscosity in apoptotic cells by two-photon fluorescence lifetime imaging

Back to tab navigation

Supplementary files

Article information


Submitted
26 Apr 2020
Accepted
03 Jun 2020
First published
03 Jun 2020

New J. Chem., 2020,44, 11342-11348
Article type
Paper

A molecular rotor sensor for detecting mitochondrial viscosity in apoptotic cells by two-photon fluorescence lifetime imaging

M. Hou, L. Liu, K. Wang, X. Chao, R. Liu and Z. Mao, New J. Chem., 2020, 44, 11342
DOI: 10.1039/D0NJ02108C

Social activity

Search articles by author

Spotlight

Advertisements