Curcumin-loaded naturally-based nanofibers as active wound dressing mats: Morphology, drug release, cell proliferation and cell adhesion studies
The perfect wound dressings should be capable of maintaining high moisture, effective exudate management, provide thermal insulation, give reliable mechanical strength. In this study, we attempted to design the desired wound dressing with curcumin (Cur) loaded electrospun zein-silk fibroin-chitosan (ZSFC) nanofiber to improve the bioavailability of Cur during the wound healing procedure. Cur with different concentrations (0.25, 0.50 and 0.75 w/v%) was loaded into the ZSFC nanofibers and successfully fabricated by electrospinning method. The SEM images of the electrospun nanofibers indicate that the mean fiber diameter altered from 176.05 ± 66.21 to 484.43 ± 176.74 nm, depending on the weight ratio (wt%) of the zein:silk fibroin:chitosan. The stiffness of the nanofibers is improved with increasing Cur concentration. Cur release investigations show a burst release at the starting stage, which is almost 45% of the Cur released in 10 hours. Besides, ZSFC and ZSFC/Cur nanofibers have enhanced the cell proliferation and adhesion, and they do not have any cytotoxic effect on human gingival fibroblast (HGF-1) cells. Fourier transform infrared (FTIR), degradation, mechanical properties, and thermal stability analysis were also performed on nanofibers. Therefore, the Cur-loaded ZSFC nanofibers results show a potential application as a wound dressing.