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A natural cyanobacterial protein C-phycoerythrin as an Hg2+ selective fluorescent probe in aqueous systems

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Abstract

A natural, yellow fluorescent cyanobacterial protein, C-phycoerythrin (CPE), was purified from a marine cyanobacterium Lyngbya sp. to a purity ratio of 5.17 using ammonium sulphate precipitation and column chromatographic techniques. After characterization by UV-visible and fluorescence spectroscopy, it was found to selectively detect micromolar quantities of Hg2+ among 12 biologically significant metal cations through the fluorescence ‘turn off’ phenomenon. Sequential titration of CPE with Hg2+ revealed a minor hypsochromic and a major hypochromic shift in its absorbance maximum and significant fluorescence quenching above 2 μM Hg2+. The response of CPE towards Hg2+ was instantaneous and unaffected by high concentrations of other cations except Cu2+. CPE was able to selectively detect and quantify Hg2+ in pond, tap and effluent water samples with recovery % of ∼94.8–107.5%. FT-IR analysis suggested Hg2+ interactions with –NH regions in the protein. Thus, CPE could be further explored as a natural agent for the selective detection of biologically and environmentally hazardous Hg2+ through the fluorescence ‘turn off’ route.

Graphical abstract: A natural cyanobacterial protein C-phycoerythrin as an Hg2+ selective fluorescent probe in aqueous systems

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Supplementary files

Article information


Submitted
01 Mar 2020
Accepted
26 Mar 2020
First published
26 Mar 2020

New J. Chem., 2020, Advance Article
Article type
Paper

A natural cyanobacterial protein C-phycoerythrin as an Hg2+ selective fluorescent probe in aqueous systems

T. Ghosh, S. Chatterjee, K. Bhayani and S. Mishra, New J. Chem., 2020, Advance Article , DOI: 10.1039/D0NJ01059F

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