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Issue 20, 2020
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A green carbon dots-based fluorescent sensor for selective and visual detection of nitrite triggered by the nitrite–thiol reaction

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Abstract

Nitrite misuse seriously threatens food safety, therefore, it is of significance to develop a facile, sensitive and selective methodology for trace detection of nitrite in food samples for ensuring people's health and safety. Herein, a novel fluorescent sensor was successfully designed for selective, sensitive and visual detection of nitrite. This sensing mechanism involves the inner-filter effect between synthetic green carbon dots and the S-nitrosothiol compound. Green carbon dots were synthesized using a one-step hydrothermal method and used as fluorescent probes, and S-nitrosothiol was generated through the π-conjugation triggered nitrite–thiol reaction. Selective and sensitive nitrite detection was realized using a fluorescence spectrophotometer in the detection range of 0.4–20 μg mL−1. The detection limit of the developed method was calculated to be 0.23 μg mL−1 with satisfactory recoveries ranging from 86.61% to 103.22%. Upon illumination with the help of a portable ultraviolet lamp, the brightness and color of the green fluorescent probe changed with the addition of nitrite at different concentrations. Visual changes were directly observed with the naked eye and used for the qualitative and semiquantitative analysis of nitrite contents. The developed fluorescent sensor offers an effective, simple, rapid and low cost analytical method for sensitive and visual nitrite detection in meat samples to monitor and reduce the risk of nitrite overuse in food processing.

Graphical abstract: A green carbon dots-based fluorescent sensor for selective and visual detection of nitrite triggered by the nitrite–thiol reaction

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Supplementary files

Article information


Submitted
28 Feb 2020
Accepted
26 Apr 2020
First published
27 Apr 2020

New J. Chem., 2020,44, 8503-8511
Article type
Paper

A green carbon dots-based fluorescent sensor for selective and visual detection of nitrite triggered by the nitrite–thiol reaction

X. Yue, Z. Zhou, Y. Wu, M. Jie, Y. Li, H. Guo and Y. Bai, New J. Chem., 2020, 44, 8503
DOI: 10.1039/D0NJ01025A

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