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Imaging of endoplasmic reticulum superoxide anion fluctuation in a liver injury model by a selective two-photon fluorescent probe

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Abstract

The superoxide anion (O2˙), with strong oxidizability and nucleophilicity, is the primary reactive oxygen species in living systems, and the overformation of O2˙ is associated with many physiological and pathological diseases. Therefore, highly sensitive and selective O2˙ detection is necessary for exploring its roles in disease situations. In this work, we presented a smart ER-targeted two-photon excitation fluorescent O2˙ probe, named TPER-O2˙, based on the caged–decaged method of characteristic hydroxyl groups on fluorophores. The TPER-O2˙ probe, with good sensitivity (detection limit: 0.33 μM) and excellent selectivity for O2˙, was applied for the detection of endogenous endoplasmic reticulum O2˙ in HepG2 cells. Furthermore, it was also successfully applied to image the endoplasmic reticulum O2˙ level fluctuation in an APAP-stimulated liver injury model. The TPER-O2˙ probe has been demonstrated to be a potential tool to unmask the role of O2˙ for endoplasmic reticulum stress-relevant clinical diseases.

Graphical abstract: Imaging of endoplasmic reticulum superoxide anion fluctuation in a liver injury model by a selective two-photon fluorescent probe

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Article information


Submitted
29 Jan 2020
Accepted
10 Mar 2020
First published
11 Mar 2020

New J. Chem., 2020, Advance Article
Article type
Paper

Imaging of endoplasmic reticulum superoxide anion fluctuation in a liver injury model by a selective two-photon fluorescent probe

Q. Zuo, Q. Wu, Y. Lv, X. Gong and D. Cheng, New J. Chem., 2020, Advance Article , DOI: 10.1039/D0NJ00487A

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