A simple chemiluminesce aptasensor for detection of α-fetoprotein based on iron-based metal organic frameworks
In this work, a sensing platform based on the luminol-H2O2-NaOH-metal organic framework (MOF) system was constructed. The relationship between the α-fetoprotein (AFP) concentration and the chemiluminescence signal was obtained with the aptamer as the target recognition element. Three iron-based metal organic frameworks (Fe-MOFs) were prepared and compared. Three Fe-MOFs with their active site showed high catalytic performances. Among Fe-MOFs, MIL-100 (Fe) held the highest catalytic activity. Thus, MIL-100 (Fe) was chosen as a peroxidase-like and was used to construct a simple chemiluminescence (CL) aptsensing platform based on the electrostatic adsorption of positive charge of MIL-100 (Fe) and negative charge of aptamer. In the presence of H2O2 and luminol, α-fetoprotein (AFP) induces aptamer conformational changes to stimulate the generation of CL. Under optimal conditions, the CL signal was linearly proportional to the AFP concentrations in the range from 1.0 × 10-10 g/L to 3.0 × 10-5 g/L. The detection limit was 7.7 × 10−11 g/L (3δ), indicating high sensitivity of this method. Undoubtedly, the reliable and easy operation of this analysis platform is a promising candidate for AFP detection in remote areas.