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A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(ii)-pyrophosphate ion chelation reaction

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Abstract

A new electrochemical immunoassay was designed for the sensitive detection of prostate-specific antigen (PSA) via a pyrophosphatase (PPase)-hydrolysed Cu(II)-coordinated pyrophosphate ion (Cu2+-PPi) complex, accompanying the release of Cu(II) ions and capture on a negatively charged electrode. This system involved a sandwiched immunoreaction with nanogold-labeled PPase/detection antibodies on a polystyrene microplate, enzymatic hydrolysis, Cu(II) release and voltammetric measurement. The voltammetric signal is derived from the as-released Cu(II) ions within the applied potential on the electrode. Under optimum conditions, our strategy exhibited a good voltammetric response for the analyte, and allowed determination of PSA at a concentration as low as 5.2 pg mL−1. Good precision, high specificity and acceptable accuracy were achieved for the detection of PSA.

Graphical abstract: A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(ii)-pyrophosphate ion chelation reaction

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Supplementary files

Article information


Submitted
05 Nov 2019
Accepted
10 Feb 2020
First published
11 Feb 2020

New J. Chem., 2020, Advance Article
Article type
Letter

A new voltammetric immunosensing platform for prostate-specific antigen based on the Cu(II)-pyrophosphate ion chelation reaction

S. Xie, B. Li, P. Lyu, H. F. Kwok, L. Ge and Q. Wu, New J. Chem., 2020, Advance Article , DOI: 10.1039/C9NJ05514B

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