Biosynthesis of graphene oxide based zinc oxide nanocomposite using Dalbergia latifolia leaf extract and its biological applications
The green synthesized zinc oxide nanoparticles (ZnO NPs) was fabricated with Graphene oxide nanosheet (hummer’s method) into ZnO/GO nanocomposite (NC) and those materials were characterized by UV-Vis, FT-IR, XRD, SEM, EDX, TEM, DLS, and zeta potential. UV-Vis spectra exhibited absorption peak around 372 nm for ZnO NPs, 233 nm for GO and 275 nm, 385 nm for ZnO/GO NC and FT-IR spectrum also confirms the formation of ZnO (457.42 cm-1), GO (1052 cm-1) and ZnO/GO NC (458.62 cm-1). XRD analysis of the synthesized nanomaterials revealed the hexagonal wurtzite structure of ZnO NPs. SEM and TEM morphological studies showed the ZnO NPs were spherical in shape, GO found to be sheet like shape and ZnO NPs immersed nanosheets were seems in ZnO/GO NC. Antibacterial effect of synthesized nanomaterial ZnO NPs, GO and ZnO/GO NC was assessed against human pathogens, significant bacterial resistance was exhibited for Staphylococcus aureus, especially ZnO/GO NC served as potent antibacterial agent. In vitro cytotoxity studies of cancer cells treated with ZnO NPs, GO and ZnO/GO NC against lung cancer cell (A549) and human breast cancer cell (MCF-7). MTT assay exhibited the significant anticancer activity of ZnO/GO NC (IC50-15 μg/mL) against MCF-7 cell line compared to other nanomaterial ZnO NPs (IC50-24 μg/mL) and GO (IC50-23 μg/mL). Thus, the study revealed that the ZnO/GO NC produced through green synthesis has great potential to be developed into an efficient therapeutic agent against various cancers.