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Resolving the internal morphology of core–shell microgels with super-resolution fluorescence microscopy

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Abstract

We investigate the internal morphology of smart core–shell microgels by super-resolution fluorescence microscopy exploiting a combination of 3D single molecule localization and structured illumination microscopy utilizing freely diffusing fluorescent dyes. This approach does not require any direct chemical labeling and does not perturb the network structure of these colloidal gels. Hence, it allows us to study the morphology of the particles with very high precision. We found that the structure of the core-forming seed particles is drastically changed by the second synthesis step necessary for making the shell, resulting in a core region with highly increased dye localization density. The present work shows that super-resolution microscopy has great potential with respect to the study of soft colloidal systems.

Graphical abstract: Resolving the internal morphology of core–shell microgels with super-resolution fluorescence microscopy

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Publication details

The article was received on 24 Oct 2019, accepted on 28 Nov 2019 and first published on 28 Nov 2019


Article type: Paper
DOI: 10.1039/C9NA00670B
Nanoscale Adv., 2020, Advance Article
  • Open access: Creative Commons BY-NC license
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    Resolving the internal morphology of core–shell microgels with super-resolution fluorescence microscopy

    P. Otto, S. Bergmann, A. Sandmeyer, M. Dirksen, O. Wrede, T. Hellweg and T. Huser, Nanoscale Adv., 2020, Advance Article , DOI: 10.1039/C9NA00670B

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