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Selenoprotein Gpx3 knockdown induces myocardial damage through Ca2+ leaks in chickens

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Abstract

Glutathione peroxidase 3 (Gpx3) is a pivotal selenoprotein that acts as an antioxidant. However, the role of Gpx3 in maintaining the normal metabolism of cardiomyocytes remains to be elucidated in more detail. Herein, we employed a model of Gpx3 interference in chicken embryos in vivo and Gpx3 knockdown chicken cardiomyocytes in vitro. Real-time PCR, western blotting and fluorescent staining were performed to detect reactive oxygen species (ROS), the calcium (Ca2+) concentration, endoplasmic reticulum (ER) stress, myocardial contraction, inflammation and heat shock proteins (HSPs). Our results revealed that Gpx3 suppression increased the level of ROS, which induced Ca2+ leakage in the cytoplasm by blocking the expression of Ca2+ channels. The imbalance of Ca2+ homeostasis triggered ER stress and blocked myocardial contraction. Furthermore, we found that Ca2+ imbalance in the cytoplasm induced severe inflammation, and HSPs might play a protective role throughout these processes. In conclusion, Gpx3 suppression induces myocardial damage through the activation of Ca2+-dependent ER stress.

Graphical abstract: Selenoprotein Gpx3 knockdown induces myocardial damage through Ca2+ leaks in chickens

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Article information


Submitted
02 Feb 2020
Accepted
18 Aug 2020
First published
02 Sep 2020

Metallomics, 2020, Advance Article
Article type
Paper

Selenoprotein Gpx3 knockdown induces myocardial damage through Ca2+ leaks in chickens

Y. Gong, J. Yang, J. Cai, Q. Liu and Z. Zhang, Metallomics, 2020, Advance Article , DOI: 10.1039/D0MT00027B

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