Jump to main content
Jump to site search


The role of necroptosis and apoptosis through the oxidative stress pathway in the liver of selenium-deficient swine

Author affiliations

Abstract

Necroptosis is regarded as a new paradigm of cell death that plays a key role in the liver damage observed with selenium (Se) deficiency. Se deficiency has a significant impact on the livestock and poultry industries. Previous studies have confirmed that Se deficiency causes serious injury to the swine liver; however, it is unclear whether this liver damage is the result of necroptosis and apoptosis. To understand the damage induced by Se deficiency, swine were divided into a control group and Se-deficient group. The results showed that in the liver of swine, Se deficiency initiated apoptosis by increasing the expression of cysteinyl aspartate specific proteinase 3 (caspase-3), cysteinyl aspartate specific proteinase 9 (caspase-9) and BCL-2 antagonist/killer (BAK) at both the mRNA and protein levels and by decreasing the B cell lymphoma/leukemia 2 (BCL-2) levels compared with the levels in the control group. Meanwhile, compared with the control group, necroptosis was confirmed in the liver of Se-deficient swine through increased the expression of mixed lineage kinase domain like pseudokinase (MLKL) and receptor interacting serine/threonine kinase 1 (RIPK1) at both the mRNA and protein levels. In addition, the activities of catalase (CAT), nitric oxide (NO), and total antioxidative capacity (T-AOC) were clearly increased (P < 0.05), and the activities of OH- and total nitric oxide synthase (TNOS) were obviously decreased (P < 0.05), whereas in the Se-deficient group, the hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels were obviously increased (P < 0.05) compared with those in the control group. Moreover, the number of apoptotic cells was increased significantly in the Se-deficient group, and the liver tissues showed obvious necroptosis damage. These results show that Se deficiency induces apoptosis and necroptosis through the oxidative stress pathway in the swine liver.

Graphical abstract: The role of necroptosis and apoptosis through the oxidative stress pathway in the liver of selenium-deficient swine

Back to tab navigation

Article information


Submitted
04 Dec 2019
Accepted
09 Mar 2020
First published
09 Mar 2020

Metallomics, 2020, Advance Article
Article type
Paper

The role of necroptosis and apoptosis through the oxidative stress pathway in the liver of selenium-deficient swine

Y. Zhang, D. Yu, J. Zhang, J. Bao, C. Tang and Z. Zhang, Metallomics, 2020, Advance Article , DOI: 10.1039/C9MT00295B

Social activity

Search articles by author

Spotlight

Advertisements