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Insight into the antitumor actions of the sterically hindered platinum(II) complex by combination of STD NMR and LCMS techniques

Abstract

The sterically hindered platinum(II) complexes have shown great advantages in overcoming the platinum drug resistance. In this study, the antitumor actions of sterically hindered platinum(II) complex 1 (cis-dichloro[(1R,2R)-N1-(2-fluorobenzyl)-1,2-diaminocyclohexane-N,N’]platinum(II), C13H19FPtCl2) was investigated by using saturation transfer difference nuclear magnetic resonance (STD NMR) and liquid chromatography–mass spectrometry (LCMS) techniques. STD NMR was applied to study the HSA (human serum albumin) binding properties, while the interactions between guanosine 5’-monophosphate (5’-GMP) and complex 1 were studied by LCMS. For the HSA binding experiments, strong STD signals were observed for protons of steric hindance parts of carrier ligands, indicating that the sterically hindered moieties of carrier ligand could be situated inside the binding pocket of HSA. 19F NMR experiment indicated that complex 1 could interact with HSA. Furthermore, the binding modes of complex 1 with guanosine 5’-monophosphate (5’-GMP) were studied in the absence and presence of glutathione by LCMS. According to the HPLC profiles, mono-functional fashion was observed for complex 1 both in the presence and absence of glutathione, while bi-adduct was observed for Pt(DACH)Cl2, which may be one of the reason for their different biological activities. Hence, this study demonstrated that NMR method combined with LCMS technique could provide valuable information to understand the transport and underlying anticnacer mechanisms of platinum(II) complex at the molecular level. Moreover, the results reported here can help to reveal the binding mechanisms of the sterically hindered platinum(II) compounds with biomolecules, which may shed light on the design of novel platinum(II) anticancer agents with suitable sterically hindered groups.

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Supplementary files

Article information


Submitted
22 Oct 2019
Accepted
14 Jan 2020
First published
14 Jan 2020

Metallomics, 2020, Accepted Manuscript
Article type
Paper

Insight into the antitumor actions of the sterically hindered platinum(II) complex by combination of STD NMR and LCMS techniques

Z. Wang, L. Fang, J. Zhao and S. Gou, Metallomics, 2020, Accepted Manuscript , DOI: 10.1039/C9MT00258H

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