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Chiral Ru(II) Complexes Act as A Potential Non-viral Gene Carrier for Directional Transportation to the Nucleus and Cytoplasm

Abstract

Guanine-rich DNA sequences can spontaneously fold into four-stranded structures which was called G-quadruplexes (G4s). G4s have been identified extensively in the promoter regions of several proto-oncogenes, including c-myc, as well as telomeres. G4s have drawn more and more attentions in fields of nanotechnology since they can be used as versatile building blocks of DNA-based nano structure. In this study,we reported the self-assembly of c-myc G-quadruplex DNA controlled by a pair of chiral ruthenium (II) complex coordinated by 2-(4-phenyacetylenephenyl)-1H-imidazo[4,5f] [1,10]phenanthroline(PBEPIP), Λ-[Ru(bpy)2(PBEPIP)](ClO4)2(Λ-RM0627), bpy=bipyridine) and Δ-[Ru(bpy)2(PBEPIP)](ClO4)2 (Δ-RM0627).It’s revealed that Λ-RM0627 can promote high-order self-assembly of c-myc G-quadruplex DNA into nanowire, while Δ-RM0627 induce DNA condensation into G-quadruplex nanoparticles. Moreover, in vitro studies on human liver carcinoma HepG2 cells show that the nano-wire of c-myc G-quadruplex DNA promoted by Λ-RM0627 can be took and localized in the nucleusof cells, whereas the nano particle of c-myc G-quadruplex DNA promoted by Δ-RM0627 can be localized in the cytoplasm. This study given a hint in enantioselectively on self-assembly of G4 DNA molecules controlled by chiral ruthenium(II) complexes and the potential applications of assembled nano-structure as non-viral carriers in gene therapy.

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Supplementary files

Article information


Submitted
29 Jul 2019
Accepted
06 Jan 2020
First published
06 Jan 2020

Metallomics, 2020, Accepted Manuscript
Article type
Paper

Chiral Ru(II) Complexes Act as A Potential Non-viral Gene Carrier for Directional Transportation to the Nucleus and Cytoplasm

Q. Wu, S. Zhang, S. Liao, J. Cao, W. Zheng, L. Li and W. Mei, Metallomics, 2020, Accepted Manuscript , DOI: 10.1039/C9MT00192A

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