Jump to main content
Jump to site search


Tuning the porosity of biofabricated chitosan membranes in microfluidics with co-assembled nanoparticles as templates

Author affiliations

Abstract

Biopolymer membranes assembled in microfluidic devices offer many biological process- and analysis-related applications. One of the key characteristics of bio-fabricated membranes is their porosity, which regulates the transport of molecules, ions, or particles and contributes to their semi-permeability and selectivity. This study aims to tune the porosity of biofabricated chitosan membranes (CM) using incorporated nanoparticles as templates. CM with polystyrene nanoparticles (CM-np) were assembled by flow in microchannel networks. The membranes with incorporated nanoparticles were crosslinked with glutaraldehyde, and then the nanoparticles were dissolved with dimethyl sulfoxide. The in situ synthesized porous CM (pCM) were characterized with scanning electron microscopy and polarized light microscopy. Permeability tests confirmed the increased pore sizes of the pCM and enhanced permeability to macromolecules. Sharper static gradients in three-channel microfluidic devices were demonstrated with the pCM as compared to those with the original CM. The capability to customize the porosity of flow-assembled, freestanding and robust biopolymer membranes inside a microfluidic network is attractive and broadens the applications of these membranes in biomolecular and cellular studies.

Graphical abstract: Tuning the porosity of biofabricated chitosan membranes in microfluidics with co-assembled nanoparticles as templates

Back to tab navigation

Supplementary files

Article information


Submitted
04 Mar 2020
Accepted
05 Mar 2020
First published
11 Mar 2020

This article is Open Access

Mater. Adv., 2020, Advance Article
Article type
Paper

Tuning the porosity of biofabricated chitosan membranes in microfluidics with co-assembled nanoparticles as templates

K. L. Ly, C. B. Raub and X. Luo, Mater. Adv., 2020, Advance Article , DOI: 10.1039/D0MA00073F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements