Issue 24, 2020

Coding and decoding stray magnetic fields for multiplexing kinetic bioassay platform

Abstract

Polymer microspheres can be fluorescently-coded for multiplexing molecular analysis, but their usage has been limited by fluorescent quenching and bleaching and crowded spectral domain with issues of cross-talks and background interference. Each bioassay step of mixing and separation of analytes and reagents require off-line particle handling procedures. Here, we report that stray magnetic fields can code and decode a collection of hierarchically-assembled beads. By the microfluidic assembling of mesoscopic superparamagnetic cores, diverse and non-volatile stray magnetic field response can be built in the series of microscopic spheres, dumbbells, pears, chains and triangles. Remarkably, the set of stray magnetic field fingerprints are readily discerned by a compact giant magnetoresistance sensor for parallelised screening of multiple distinctive pathogenic DNAs. This opens up the magneto-multiplexing opportunity and could enable streamlined assays to incorporate magneto-mixing, washing, enrichment and separation of analytes. This strategy therefore suggests a potential point-of-care testing solution for efficient kinetic assays.

Graphical abstract: Coding and decoding stray magnetic fields for multiplexing kinetic bioassay platform

Supplementary files

Article information

Article type
Paper
Submitted
20 Aug 2020
Accepted
25 Oct 2020
First published
04 Nov 2020

Lab Chip, 2020,20, 4561-4571

Coding and decoding stray magnetic fields for multiplexing kinetic bioassay platform

Y. Liu, G. Lin, Y. Chen, I. Mönch, D. Makarov, B. J. Walsh and D. Jin, Lab Chip, 2020, 20, 4561 DOI: 10.1039/D0LC00848F

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