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Issue 17, 2020
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Label-free ferrohydrodynamic separation of exosome-like nanoparticles

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Abstract

Isolation of exosomes from biological samples provides a minimally-invasive alternative for basic understanding, diagnosis, and prognosis of metastatic cancers. The biology and clinical values of exosomes are under intensive investigation, yet most studies are limited by technical challenges in recovering these exosomes with heterogeneous sizes and cargos from biological samples. We report a novel method based on “particle ferrohydrodynamics” and its associated microfluidic device, termed as the FerroChip, which can separate exosome-like nanoparticles from microliters of cell culture media and human serum in a label-free, continuous-flow and size-dependent manner, and achieves a high recovery rate (94.3%) and a high purity (87.9%). Separated exosome-like nanoparticles had diameters, morphology, and protein expressions that were consistent with other reports. This method, upon further molecular characterization, could potentially facilitate basic understanding of exosomes and its clinical application in blood liquid biopsy.

Graphical abstract: Label-free ferrohydrodynamic separation of exosome-like nanoparticles

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Supplementary files

Article information


Submitted
12 Jun 2020
Accepted
21 Jul 2020
First published
24 Jul 2020

Lab Chip, 2020,20, 3187-3201
Article type
Paper

Label-free ferrohydrodynamic separation of exosome-like nanoparticles

Y. Liu, W. Zhao, R. Cheng, M. Logun, M. D. M. Zayas-Viera, L. Karumbaiah and L. Mao, Lab Chip, 2020, 20, 3187
DOI: 10.1039/D0LC00609B

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