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ECM-based microchannel for culturing in vitro vascular tissues with simultaneous perfusion and stretch

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Abstract

We present an extracellular matrix (ECM)-based stretchable microfluidic system for culturing in vitro three-dimensional (3D) vascular tissues, which mimics in vivo blood vessels. Human umbilical vein endothelial cells (HUVECs) can be cultured under perfusion and stretch simultaneously with real-time imaging by our proposed system. Our ECM (transglutaminase (TG) cross-linked gelatin)-based microchannel was fabricated by dissolving water-soluble sacrificial polyvinyl alcohol (PVA) molds printed with a 3D printer. Flows in the microchannel were analyzed under perfusion and stretch. We demonstrated simultaneous perfusion and stretch of TG gelatin-based microchannels culturing HUVECs. We suggest that our TG gelatin-based stretchable microfluidic system proves to be a useful tool for understanding the mechanisms of vascular tissue formation and mechanotransduction.

Graphical abstract: ECM-based microchannel for culturing in vitro vascular tissues with simultaneous perfusion and stretch

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Supplementary files

Article information


Submitted
12 Mar 2020
Accepted
11 Apr 2020
First published
20 Apr 2020

Lab Chip, 2020, Advance Article
Article type
Paper

ECM-based microchannel for culturing in vitro vascular tissues with simultaneous perfusion and stretch

A. Shimizu, W. H. Goh, S. Itai, M. Hashimoto, S. Miura and H. Onoe, Lab Chip, 2020, Advance Article , DOI: 10.1039/D0LC00254B

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