Jump to main content
Jump to site search

Issue 7, 2020
Previous Article Next Article

A microfluidic approach for experimentally modelling the intercellular coupling system of a mammalian circadian clock at single-cell level

Author affiliations

Abstract

In mammals, it is believed that the intercellular coupling mechanism between neurons in the suprachiasmatic nucleus (SCN) confers robustness and distinguishes the central clock from peripheral circadian oscillators. Current in vitro culturing methods used in Petri dishes to study intercellular coupling by exogenous factors invariably cause perturbations, such as simple media changes. Here, we design a microfluidic device to quantitatively study the intercellular coupling mechanism of circadian clock at the single cell level, and demonstrate that vasoactive intestinal peptide (VIP) induced coupling in clock mutant Cry1-/- mouse adult fibroblasts engineered to express the VIP receptor, VPAC2, is sufficient to synchronize and maintain robust circadian oscillations. Our study provides a proof-of-concept platform to reconstitute the intercellular coupling system of the central clock using uncoupled, single fibroblast cells in vitro, to mimic SCN slice cultures ex vivo and mouse behavior in vivo phenotypically. Such a versatile microfluidic platform may greatly facilitate the studies of intercellular regulation networks, and provide new insights into the coupling mechanisms of the circadian clock.

Graphical abstract: A microfluidic approach for experimentally modelling the intercellular coupling system of a mammalian circadian clock at single-cell level

Back to tab navigation

Supplementary files

Article information


Submitted
10 Feb 2020
Accepted
28 Feb 2020
First published
02 Mar 2020

Lab Chip, 2020,20, 1204-1211
Article type
Paper

A microfluidic approach for experimentally modelling the intercellular coupling system of a mammalian circadian clock at single-cell level

K. Han, L. Mei, R. Zhong, Y. Pang, E. E. Zhang and Y. Huang, Lab Chip, 2020, 20, 1204
DOI: 10.1039/D0LC00140F

Social activity

Search articles by author

Spotlight

Advertisements