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Issue 6, 2020
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Antibody-coated microstructures for selective isolation of immune cells in blood

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Abstract

Cell isolation from blood is an important process for diagnosing immune diseases. There are still demands for a user-friendly approach to achieve high cell extraction efficiency and purity of a target immune cell subtype for more promising diagnosis and monitoring. For selective immune cell isolation, we developed a microstructured device, which consists of antibody-coated micropillars and micro-sieve arrays, for isolating a target immune cell subtype from bovine blood samples. The focusing micropillars can guide immune cells flowing to the subsequent micro-sieves based on deterministic lateral shifts of the cells. The arrangement of these microstructures is characterized and configured for the maximal cell capture rate. Surface modification with a selected antibody offers selective cell capture in the micro-sieves based on the antigen–antibody reaction. We prepare a cell mixture of human CD14-expressing leukemia cells (THP-1) and epithelial cells (MDA-MB-231) in diluted blood to characterize the cell isolation operation, with a selective cell isolation yield of >80%, cell purity of ∼100% and cell viability of >93%. Together, this microstructured device strategy can achieve high-yield selective isolation of immune cells from blood samples and support downstream genetic and biochemical cell analyses, contributing to the medical diagnosis of a broad range of immune diseases.

Graphical abstract: Antibody-coated microstructures for selective isolation of immune cells in blood

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Supplementary files

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Submitted
23 Jan 2020
Accepted
09 Feb 2020
First published
13 Feb 2020

Lab Chip, 2020,20, 1072-1082
Article type
Paper

Antibody-coated microstructures for selective isolation of immune cells in blood

J. Li, Y. Liu, J. Ren, B. Z. Tay, T. Luo, L. Fan, D. Sun, G. Luo, D. Lau, Marcos and R. H. W. Lam, Lab Chip, 2020, 20, 1072
DOI: 10.1039/D0LC00078G

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