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Surfactant and oil formulations for monodisperse droplet emulsion PCR

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Abstract

Emulsion PCR has become a popular and widely applied method in biological research and clinical diagnostics to provide evenly amplified products and perform highly quantitative counting of target sequences. However, there is still a lack of information to support further development of appropriate water-in-oil emulsion formulations, which need to be both thermally and mechanically stable for digital amplification reactions. Here, we present a systematic survey of the oil and surfactant components of stable monodisperse w/o emulsions suitable for use with our previously developed micro-capillary array (MiCA)-based centrifugal emulsion generation method. Our findings show that a binary formula consisting of isopropyl palmitate and a silicone copolymer demonstrated the best performance, and provided a general guideline for the development of emulsion systems for digital PCR and emulsion amplification applications.

Graphical abstract: Surfactant and oil formulations for monodisperse droplet emulsion PCR

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Supplementary files

Article information


Submitted
16 Jan 2020
Accepted
15 May 2020
First published
15 May 2020

Lab Chip, 2020, Advance Article
Article type
Paper

Surfactant and oil formulations for monodisperse droplet emulsion PCR

F. Zhang, P. Liao, Y. Sun, Z. Chen, Y. Pang and Y. Huang, Lab Chip, 2020, Advance Article , DOI: 10.1039/D0LC00052C

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