Fully automated platelet isolation on a centrifugal microfluidic device for molecular diagnostics
Platelets play crucial roles in hemostasis and immunity. Over the last decades, clinical evidence has revealed the significance of platelets as substitutionary biomarkers for the detection and treatment of various diseases, including cancer. Due to a lack of well standardized convenient isolation method of platelets, pre-analytical factors such as complex handling procedures negatively impact the quality of the platelet samples, including overactivation, low purity, and poor reproducibility. This may lead to biased interpretation of various downstream analyses, such as proteomics and genomics analyses. Herein, we describe a fully automated lab-on-a-disc–based method of platelet isolation from a small volume of blood (<1 mL). This method provides higher yields (>4 folds) and purity (>99%), and lower platelet activation than the conventional method. Moreover, it was also superior in the detection of the platelet-related RNAs CD41, PF4, and P2Y12 due to lower contamination with white blood cells.