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Infrared spectroscopy of live cells from a flowing solution using electrically-biased plasmonic metasurfaces

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Abstract

Spectral cytopathology (SCP) is a promising label-free technique for diagnosing diseases and monitoring therapeutic outcomes using FTIR spectroscopy. In most cases, cells must be immobilized on a substrate prior to spectroscopic interrogation. This creates significant limitations for high throughput phenotypic whole-cell analysis, especially for the non-adherent cells. Here we demonstrate how metasurface-enhanced infrared reflection spectroscopy (MEIRS) can be applied to a continuous flow of live cell solution by applying AC voltage to metallic metasurfaces. By integrating metasurfaces with microfluidic delivery channels and attracting the cells to the metasurface via dielectrophoretic (DEP) force, we collect the infrared spectra of cells in real time within a minute, and correlate the spectra with simultaneously acquired images of the attracted cells. The resulting DEP-MEIRS technique paves the way for rapid SCP of complex cell-containing body fluids with low cell concentrations, and for the development of a wide range of label-free liquid biopsies.

Graphical abstract: Infrared spectroscopy of live cells from a flowing solution using electrically-biased plasmonic metasurfaces

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Supplementary files

Article information


Submitted
24 Oct 2019
Accepted
03 May 2020
First published
14 May 2020

Lab Chip, 2020, Advance Article
Article type
Paper

Infrared spectroscopy of live cells from a flowing solution using electrically-biased plasmonic metasurfaces

G. Kelp, J. Li, J. Lu, N. DiNapoli, R. Delgado, C. Liu, D. Fan, S. Dutta-Gupta and G. Shvets, Lab Chip, 2020, Advance Article , DOI: 10.1039/C9LC01054H

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