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An integrated microfluidic system for rapid, automatic and high-throughput staining of clinical tissue samples for diagnosis of ovarian cancer

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Abstract

Accurate cancer diagnostic methods are of urgent need. Since traditional immunohistochemistry (IHC)-based approaches, while reliable, are labor-intensive and require well-trained technicians, we developed an integrated microfluidic platform capable of labeling ovarian cancer biomarkers (i.e. aptamer) within formalin-fixed, paraffin embedded tissues via molecular probes. Both aptamer-based 1) fluorescent staining and 2) IHC staining of clinical tissue samples could be automated in the microfluidic system in only 2–3 h (40–50% faster than conventional approaches) with <0.5 mL of reagents, signifying that this device could serve as a promising diagnostic tool for ovarian cancer.

Graphical abstract: An integrated microfluidic system for rapid, automatic and high-throughput staining of clinical tissue samples for diagnosis of ovarian cancer

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Article information


Submitted
01 Oct 2019
Accepted
28 Jan 2020
First published
30 Jan 2020

Lab Chip, 2020, Advance Article
Article type
Paper

An integrated microfluidic system for rapid, automatic and high-throughput staining of clinical tissue samples for diagnosis of ovarian cancer

S. Huang, Y. Chuang, W. Lee, Y. Tsai, C. Lin, K. Hsu and G. Lee, Lab Chip, 2020, Advance Article , DOI: 10.1039/C9LC00979E

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