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Issue 1, 2020
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Microfluidic system for Caenorhabditis elegans culture and oxygen consumption rate measurements

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Abstract

Mitochondrial respiration is a key signature for the assessment of mitochondrial functioning and mitochondrial dysfunction is related to many diseases including metabolic syndrome and aging-associated conditions. Here, we present a microfluidic Caenorhabditis elegans culture system with integrated luminescence-based oxygen sensing. The material used for the fabrication of the microfluidic chip is off-stoichiometry dual-cure thiol–ene–epoxy (OSTE+), which is well-suited for reliably recording on-chip oxygen consumption rates (OCR) due to its low gas permeability. With our microfluidic approach, it was possible to confine a single nematode in a culture chamber, starting from the L4 stage and studying it over a time span of up to 6 days. An automated protocol for successive worm feeding and OCR measurements during worm development was applied. We found an increase of OCR values from the L4 larval stage to adulthood, and a continuous decrease as the worm further ages. In addition, we performed a C. elegans metabolic assay in which exposure to the mitochondrial uncoupling agent FCCP increased the OCR by a factor of about two compared to basal respiration rates. Subsequent treatment with sodium azide inhibited completely mitochondrial respiration.

Graphical abstract: Microfluidic system for Caenorhabditis elegans culture and oxygen consumption rate measurements

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Article information


Submitted
20 Aug 2019
Accepted
30 Oct 2019
First published
15 Nov 2019

Lab Chip, 2020,20, 126-135
Article type
Paper

Microfluidic system for Caenorhabditis elegans culture and oxygen consumption rate measurements

R. Krenger, M. Cornaglia, T. Lehnert and M. A. M. Gijs, Lab Chip, 2020, 20, 126
DOI: 10.1039/C9LC00829B

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