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Molecular absorption and mass spectrometry for complementary analytical study of fluorinated drugs in animal organisms

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Abstract

Although several analytical techniques are known for their ability to detect fluorine and its compounds, the investigation of its biotransformation in living organisms is not a trivial task. Therefore, much attention is given to the development of new analytical strategies that can trace the metabolism of fluorine-containing compounds. Molecular absorption and mass spectrometry were used as tools to collect complementary information for the investigation of the biotransformation of the fluorine-containing compounds in animal model organisms. The study aimed to examine the effectiveness of the adsorption of fluorine by animal tissues, which was done by measuring the molecular absorption of gallium monofluoride (GaF) using high-resolution continuum source graphite furnace absorption spectrometry (HR-CS GF MAS). The limit of detection (LOD) was 6 μg L−1. Then, the distribution of fluorine-containing compunds was obtained by measuring the studied drug concentrations in animal tissues using high-performance liquid chromatography with electrospray ionisation (ESI) tandem mass spectrometry (HPLC-ESI-MS/MS). The LOD for compounds containing fluorine was 0.05 μg kg−1. Finally, proteins containing fluorine modifications bound in a covalent way were studied using ultra-high performance liquid chromatography coupled to an electrospray mass spectrometer equipped with a high resolution Orbitrap mass analyzer (nano-UHPLC-ESI-(Orbitrap)-MS/MS). The developed analytical protocol contributes to considerable progress in understanding the molecular basis of the influence of fluorine-containing compounds on aminal organisms.

Graphical abstract: Molecular absorption and mass spectrometry for complementary analytical study of fluorinated drugs in animal organisms

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Article information


Submitted
29 Mar 2020
Accepted
08 Jun 2020
First published
23 Jun 2020

J. Anal. At. Spectrom., 2020, Advance Article
Article type
Paper

Molecular absorption and mass spectrometry for complementary analytical study of fluorinated drugs in animal organisms

A. Gawor, A. Konopka, A. Ruszczyńska, L. Pączek, Z. Gajewski and E. Bulska, J. Anal. At. Spectrom., 2020, Advance Article , DOI: 10.1039/D0JA00126K

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