Jump to main content
Jump to site search

Issue 17, 2020

Enrichment of glycopeptides using environmentally friendly wood materials

Author affiliations

Abstract

Glycoproteomics is one of the main routes to study protein post-translational modifications, and enrichment is one of the important steps. Many hydrophilic materials have been used for glycopeptide enrichment, however involving complex synthesis procedures, high consumption in cost, time, and chemicals. Here, balsa wood, a naturally available hydrophilic material with highly porous structure, was investigated for immunoglobulin G (IgG) glycopeptide enrichment in a micropipette set-up. Native balsa wood without any pretreatment provided a green alternative to a commercial HILIC product, exhibiting comparable, or even better, enrichment performance for IgG standard and human plasma IgG samples. After delignification, binding capacity and recovery could be improved due to increased hydrophilicity and porosity. The developed method utilizing wood materials introduced an environmentally friendly option for glycoproteomics, saving both costs and chemicals, while exhibiting high performance.

Graphical abstract: Enrichment of glycopeptides using environmentally friendly wood materials

Supplementary files

Article information


Submitted
28 Apr 2020
Accepted
30 Jul 2020
First published
31 Jul 2020

This article is Open Access

Green Chem., 2020,22, 5666-5676
Article type
Paper

Enrichment of glycopeptides using environmentally friendly wood materials

Y. Zhou, X. Sheng, J. Garemark, L. Josefsson, L. Sun, Y. Li and Å. Emmer, Green Chem., 2020, 22, 5666 DOI: 10.1039/D0GC01467B

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements