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Issue 13, 2020
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Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions

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Abstract

The development of biocatalytic routes for the synthesis of chiral amines starting from achiral building blocks is highly desirable. Here, we report a self-sufficient whole-cell system for the conversion of a model ketone to the corresponding cyclic imine, in good isolated yield (42%) and excellent enantioselectivity (>99% ee). The Corynebacterium glutamicum host produces the transaminase biocatalyst, cofactor and ‘smart’ amine donor (cadaverine or putrescine) in vivo, and highlights the potential for producing high-value chemicals from readily available building blocks. The report represents the first example of the application of a metabolically engineered organism for the production of smart diamine donors and their application in a transaminase biotransformation.

Graphical abstract: Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions

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Article information


Submitted
02 May 2020
Accepted
08 Jun 2020
First published
11 Jun 2020

Green Chem., 2020,22, 4128-4132
Article type
Communication

Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions

S. Grigoriou, P. Kugler, E. Kulcinskaja, F. Walter, J. King, P. Hill, V. F. Wendisch and E. O'Reilly, Green Chem., 2020, 22, 4128
DOI: 10.1039/D0GC01432J

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