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Production of formate from CO2 gas under ambient conditions: towards flow-through enzymes reactors

Abstract

A flow-through bi-enzymatic reactor was developed to produce formate from CO2 gas. The reaction operates under ambient conditions without H2 and involves a high performance formate dehydrogenase from Methylobacterium extorquens AM1 (MeFoDH1). The tandem reaction comprises a clean in situ regeneration of the nicotinamide adenine dinucleotide (1,4-NADH) cofactor with a highly active mutant phosphite dehydrogenase from Pseudomonas stutzeri, giving no side reactions. The highest turnover number was reached (6 700) giving a high space-time yield of 0.6 mmolformate min-1 L-1reactor.

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Supplementary files

Article information


Submitted
17 Mar 2020
Accepted
15 May 2020
First published
15 May 2020

Green Chem., 2020, Accepted Manuscript
Article type
Communication

Production of formate from CO2 gas under ambient conditions: towards flow-through enzymes reactors

N. Brun, J. Drone, A. Galarneau, K. Kano, A. Mehdi, E. Dubreucq, A. Lamotte, M. Baccour and K. Sakai, Green Chem., 2020, Accepted Manuscript , DOI: 10.1039/D0GC00952K

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