Cytochrome c-Urea Functionalized Dipeptide Conjugate: An Efficient HBD framework, to synthesize 4H-Pyrans via One-Pot Multicomponent Reaction
This work is focused on the development of an efficient and green protocol for the one-pot multicomponent synthesis of a series of 4H-Pyran derivatives. Herein, Protein- Peptideconjugate (SS1-Cyt.c) is synthesized and characterized using Circular Dichroism (CD), UV-Visible absorption spectroscopy and Docking studies. The whole assembly is used as an efficient catalytic system by incorporating a derivatized dipeptide molecule in the hydrophobic cavities of the protein Cytochrome c (Cyt. c) that leads to the activation of Hydrogen Bond Donating (HBD) units of urea derivative of a dipeptide (SS1). The HBD units tend to show non-covalent interaction with the reactant species responsible for triggering the organic transformation to take place. Also, the stability and binding interactions of the SS1-Cyt.c with the multicomponent reactants involved in the organic transformation is studied in detail using MD-Simulation studies. It is a metal-free catalyst and provided higher yield in relatively short reaction time. As per green chemistry premises, the catalyst has provided us ideal Eco-scale and E factor score of 79 and 0.089 respectively.